ERAMUC-1

General#

Cell Line

hPSCreg Name
ULBe004-A
Alternative name(s)
ERAMUC-1
Cell line type Human embryonic stem cell (hESC)
Last update 5th March 2021
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Provider

Generator Université Libre de Bruxelles (ULB)
Distributors
Derivation country Belgium

External Databases

Cellosaurus CVCL_9008
CLO CLO_0103254
Wikidata Q54990725

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Cystic Fibrosis
The donor is affected.
Synonyms
  • Cystic Fibrosis
  • cystic fibrosis
Disease associated phenotypes
  • Monogenetic Disease

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Federal Commission for Medical and Scientific Research on embryos in vitro
Approval number

hESC Derivation#

Date of derivation 2006-02-14
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Embryo

Culture Conditions#

Surface coating Gelatin
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source:
Serum concentration: 15 %
Supplements
glutamax

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
hTERT
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
69, XXX
Passage number: 20

Other Genotyping (Cell Line)