ERAMUC-1
ULBe004-A
General
Cell Line |
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| hPSCreg Name | ULBe004-A |
| Alternative name(s) |
ERAMUC-1
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 4th September 2022 |
| User feedback | |
Provider |
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| Generator | Université Libre de Bruxelles (ULB) |
| Derivation country | Belgium |
External Databases |
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| Cellosaurus | CVCL_9008 |
| CLO | CLO_0103254 |
| Wikidata | Q54990725 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
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Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Federal Commission for Medical and Scientific Research on embryos in vitro |
| Approval number |
hESC Derivation
| Date of derivation | 2006-02-14 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
Yes |
| ZP removal technique | Chemical |
| Cell isolation | Mechanical |
| Cell seeding | Embryo |
Culture Conditions
| Surface coating | Gelatin | ||||||
| Passage method | Mechanically | ||||||
| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Serum concentration: 15 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| hTERT |
Yes |
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| NANOG |
Yes |
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| ZFP42 (REX-1) |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
69, XXX
Passage number: 20
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Other Genotyping (Cell Line) |
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