HAD 2

General#

Cell Line

hPSCreg Name HADe002-A
Alternative name(s)
HAD 2
Cell line type Human embryonic stem cell (hESC)
Last update 27th October 2017
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Provider

Generator Hadassah University Hospital (HAD)
Derivation country Israel

External Databases

Cellosaurus CVCL_C073

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Cystic fibrosis
The donor is affected.
Synonyms
  • Mucoviscidosis
  • CF

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
PGD Embryo?
Yes
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Spontaneous Hatching
Cell isolation Laser
Cell seeding Isolated ICM

Culture Conditions#

Surface coating Gelatin
Feeder cells human foreskin fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Enzymatically
Collagenase
O2 Concentration 4 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
hrbFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
beta Tubulin-3
Yes
Desmin
Yes
sox-17
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)