Phx_SRCAP_g3_1400_6/8
The cell line is not validated yet.
MHHi001-A-14
General
Cell Line |
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| hPSCreg name | MHHi001-A-14 |
| Cite as: | MHHi001-A-14 |
| Alternative name(s) |
Phx_SRCAP_g3_1400_6/8
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 23rd May 2025 |
| User feedback | |
Provider |
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| Generator | Department of Functional Genomics - Human Molecular Genetics (UMGW) |
External Databases |
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| BioSamples | SAMEA114222274 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | N/A |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA4564585 |
Ethics
Also have a look at the ethics information for the parental line
MHHi001-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
MHHi001-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
Immunostaining, RT-PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | Sanger sequencing for mutations in target region Frameshift analysed using online tools TIDE (https://tide.nki.nl/) and indigo (https://www.gear-genomics.com/indigo/) |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
No |
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| Passage method | Mechanically | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
mTeSR™ 1
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| TRA 1-60 |
Yes |
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
Morphology
Protocol or reference
In vitro spontaneous differentiation
Morphology
Protocol or reference
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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