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MHHi001-A-12

Registration Summary:

Phx_SRCAP_g3_1200_4

MHHi001-A-12

General

Cell Line
hPSCreg name MHHi001-A-12
Cite as:
MHHi001-A-12 (RRID:CVCL_D6KV)
Alternative name(s)
Phx_SRCAP_g3_1200_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MHHi001-A-13
(Phx_SRCAP_g3_1200_7)
MHHi001-A
(hHSC_Iso4_ADCF_SeViPS2 (Phönix))
MHHi001-A-7
(NRF2_A_Phönix-iPSC clone19)
MHHi001-A-6
(NRF2_A_Phönix-iPSC clone12)
Last update 27th September 2023
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Provider
Generator Department of Functional Genomics - Human Molecular Genetics (UMGW)
Owner Department of Functional Genomics - Human Molecular Genetics (UMGW)
Derivation country Germany

External Databases
BioSamples SAMEA114222272
Cellosaurus CVCL_D6KV
Wikidata Q127382826

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)
Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)
BioSamples SAMEA4564585

Ethics

Also have a look at the ethics information for the parental line MHHi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General
The source cell information can be found in the parental cell line MHHi001-A.

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection rtPCR with Primer specific for genes on SeV vector and vector backbone, GAPDH as positive control
Files and images showing reprogramming vector expressed or silenced
vector_4.png

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Supplements
Penicillin-Streptomycin, 10.000 U/ml Penicillin, 10 mg/ml Pan Biotech 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
–
–
SOX2
Yes
–
–
TRA 1-60
Yes
–
NANOG
Yes
–
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Morphology
Embryoid body shows expression of AFP
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
Morphology
Embryoid body shows expression of SMA
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
Morphology
Embryoid body shows expression of TUJ1

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46XX
Karyotype_A-12.png
Karyotyping method: low-coverage whole genome sequencing (lcWGS)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Genetic modifications
SRCAP (target)
Isogenic modification
16p11.2
NC_000016.10:g.[30739347_30739348del];[30739347del]
NP_006653.2:p.[Leu3104TrpfsTer3];[Gly3103ValfsTer31]
Heterozygous
Mutation was introduced by CRISPR/Cas9 RNP Sanger Sequencing in forward and reverse direction was done on the target region. InDel length and frequency was estimated with TIDE (http://shinyapps.datacurators.nl/tide/). InDel length and allelic deconvolution was performed using Indigo (https://www.gear-genomics.com/indigo/, Gear Genomics). Both tools agree on the InDel lengths leading to truncating mutations on both alleles.
Mutated