Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242
Summary
Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of electrophysiological properties of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). A dual transgenic hiPSC line with Arclight A242 (GEVI) and an antibiotic resistance cardiac selection cassette was successfully generated from an earlier established hiPSC line MHHi001-A. After cardiac differentiation and selection, purified populations of CMs with constitutive GEVI expression can be utilized for studying cardiac development, disease modeling, and drug testing. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Vivekanandan R, Szepes M, Ricci Signorini ME, Kravchenko D, Kiefer J, Berger S, Fricke V, Göhring G, Gruh I |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Feb;66:102981 |
| PubMed | 36463634 |
| DOI | 10.1016/j.scr.2022.102981 |