CABi001-A, PRPF31-MiPS4F3

General#

Cell Line

hPSCreg Name
ESi077-A
Alternative name(s)
CABi001-A, PRPF31-MiPS4F3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th September 2020
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Provider

Generator Spanish Stem Cell Bank (ES)
Distributors

External Databases

Cellosaurus CVCL_UJ71
BioSamples SAMEA7484330
CLO CLO_0103058
Wikidata Q93545418

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis Pigmentosa
The donor is a carrier of a disease-associated mutation and affected.
Stage
Advanced
Synonyms
  • Retinitis Pigmentosa
Genetic variants
PRPF31 (target)
Chromosome 19: 54,115,410-54,131,719 forward strand.
Heterozygous

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

All cell lines of this donor's relatives
Has son:

External Databases (Donor)

BioSamples SAMEA7484331

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: berta.delacerda@cabimer.es
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Comité Coordinador de Ética de a Investigación Biomédica de Andalucía
Approval number PR-01-2015
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Comité Coordinador de Ética de a Investigación Biomédica de Andalucía
Approval number PR-01-2015
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Derived Peripheral Blood Mononuclear Cell
Mononuclear cells collected from peripheral blood.
Synonyms
  • Peripheral Blood Mononuclear cells
  • Derived Peripheral Blood Mononuclear Cell
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection rtPCR to detect ectopic reprogramming factors and capsid gene. c+ is PRPF31-MiPS4F3 at passage 1 and c- is a primary cell sample (PBMCs). Third lane shows the silencing of the virus in the PRPF31-MiPS4F3 line at passage 6
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones Manually picking iPS clones with ESC-morphology and positive for in vivo TRA-1-60 immunofluorescent staining.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SOX2
Yes
TRA 1-60
Yes
SSEA-3
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Marker Expressed
Alpha-fetoprotein
Yes
Morphology
AFP
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Marker Expressed
Actin Smooth muscle
Yes
Morphology
Actin Smooth Muscle
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Marker Expressed
TUBB3
Unknown
Morphology
TUBB3

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 13
Karyotyping method: G-Banding

Other Genotyping (Cell Line)