RP003C12

General

Cell Line

hPSCreg name NUIGi029-A
Cite as:
NUIGi029-A (RRID:CVCL_YC19)
Alternative name(s)
RP003C12
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
LUMCi056-A-1
(LUMC0128iCRB01 heterozygote CRISPR corrected isogenic clone 02, iso02LUMC0128iCRB01)
Donor's gene variants:
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Retinitis Pigmentosa
FRIMOi001-A
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LUMCi055-A
(CRB1 patient 117 compound heterozygous 2983G>T p.(Glu995*) c.1892A>G, p.(Tyr631Cys), LUMC0117iCRB01)
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Retinitis Pigmentosa
NUIGi027-A
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(EYS mutation)
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retinitis pigmentosa
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retinitis pigmentosa
LUMCi056-A
(CRB1 patient 128 compound heterozygous c.2843G>A p.(Cys948Tyr) and c.3122T>C p.(Met1041Thr), LUMC0128iCRB01)
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CRB1, CRB1
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Retinitis Pigmentosa
CABi002-A
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retinitis pigmentosa
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(HPSI0816i-oemk_6)
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UNEWi004-A
(PRPF31 SH)
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PRPF31
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UNEWi005-A
(PRPF31 RH)
Donor's gene variants:
PRPF31
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Retinitis pigmentosa
WTSIi463-A
(HPSI0716i-yibs_1)
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(CW70323)
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retinitis pigmentosa
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WTSIi610-A
(HPSI1116i-naum_5)
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WTSIi691-A
(HPSI1116i-eiyy_1)
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WTSIi693-A
(HPSI1116i-zies_2)
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Retinitis pigmentosa
UNEWi001-A
(UNEW001Ai)
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PRPF31
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UNEWi002-A
(UNEW002Ai, PRPF31 AW)
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PRPF31, PRPF31
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Retinitis pigmentosa
UNEWi003-A
(PRPF31-HD)
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Retinitis pigmentosa
WTSIi698-A
(HPSI1116i-aimh_5)
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WTSIi713-A
(HPSI0816i-xoga_7)
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RIi009-A
(RP2.H.iPSC.3)
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(HPSI1116i-aimh_3)
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WTSIi613-B
(HPSI1116i-rafd_1)
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Retinitis pigmentosa
WTSIi613-A
(HPSI1116i-rafd_4)
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Retinitis pigmentosa
ESi077-A
(CABi001-A, PRPF31-MiPS4F3)
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PRPF31, PRPF31
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Retinitis Pigmentosa
CIRMi525-A
(CW70348)
Donor diseases:
retinitis pigmentosa
UNEWi027-A
(F116)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
WTSIi713-B
(HPSI0816i-xoga_12)
Donor diseases:
Retinitis pigmentosa
WTSIi688-A
(HPSI1116i-wiau_1)
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Retinitis pigmentosa
RIi012-A
(RP.1.H.iPSC.2)
Donor diseases:
Retinitis pigmentosa
Last update 6th November 2019
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Provider

Generator National University of Ireland Galway (NUIG)

External Databases

BioSamples SAMEA6348712
Cellosaurus CVCL_YC19
Wikidata Q98128194

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Retinitis Pigmentosa

External Databases (Donor)

BioSamples SAMEA6348713

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Essential 8™ Flex

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
SNP genotyping

Other Genotyping (Cell Line)