LEIi004-A-1

General#

Cell Line

hPSCreg Name LEIi004-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd October 2018
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Provider

Generator Lions Eye Institute (LEI)
Owner Lions Eye Institute (LEI)

External Databases

BioSamples SAMEA4760675
Cellosaurus CVCL_VE94

General Information

Publications
Is the cell line available in principle?
No
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis pigmentosa
Family history RCD , CLN3 mutation
Is the medical history available upon request? YES
Is clinical information available? YES

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104626353

Ethics#

Also have a look at the ethics information for the parental line LEIi004-A .
Alternatives to consent
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? SBI Cat#SC900A-1
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No
Constraints for use or distribution

hIPSC Derivation#

General

The source cell information can be found in the parental cell line LEIi004-A.
Passage number reprogrammed 25

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Retinitis pigmentosa
Genetic modifications
Variant
16p12.1
NP_001035897 c.175G>A
Heterozygous