LEIi004-A-1

General#

Cell Line

hPSCreg Name LEIi004-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd October 2018
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Provider

Generator Lions Eye Institute (LEI)
Owner Lions Eye Institute (LEI)

External Databases

BioSamples SAMEA4760675
Cellosaurus CVCL_VE94

General Information

Publications
Is the cell line available in principle?
No
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis pigmentosa
Family history RCD , CLN3 mutation
Is the medical history available upon request? YES
Is clinical information available? YES

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104626353

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line LEIi004-A.
Passage number reprogrammed 25

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Retinitis pigmentosa
Genetic modifications
Variant
16p12.1
NP_001035897 c.175G>A
Heterozygous