General#

Cell Line

hPSCreg Name LEIi004-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd October 2018
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Provider

Generator Lions Eye Institute (LEI)
Owner Lions Eye Institute (LEI)
Derivation country Australia

External Databases

BioSamples SAMEA104626352
Cellosaurus CVCL_VE61

General Information

Publications
Is the cell line available in principle?
No
Subclones

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis pigmentosa
Disease associated phenotypes
  • Retinitis Pigmentosa
Family history RCD , CLN3 mutation
Is the medical history available upon request? YES
Is clinical information available? YES

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104626353

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Passage number reprogrammed 7

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46+XX

Other Genotyping (Cell Line)