KLRMMEi001-A

General#

Cell Line

hPSCreg Name KLRMMEi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 10th August 2018
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Provider

Generator Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University. (KLRMME)
Owner Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University. (KLRMME)
Derivation country China

External Databases

BioSamples SAMEA104626790
Cellosaurus CVCL_VE03

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis pigmentosa
The donor is a carrier of a disease-associated mutation.
Genetic variants
Homozygous
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104626791

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
olfactory epithelium
A sensory epithelium inside the nasal cavity that is responsible for detecting odors[WP].
Synonyms
  • olfactory sensory epithelium
  • sensory olfactory epithelium
  • olfactory membrane
  • main olfactory epithelium
  • nasal cavity olfactory epithelium
show more synonyms
Source cell origin
urine
Excretion that is the output of a kidney
Age of donor (at collection) 25-29
Collected in 2017
Passage number reprogrammed 1

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Yes
Methods used
Immunostaining, PCR, RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
In vitro three germ layer differentiation immunofluorescence staining
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro spontaneous differentiation
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro spontaneous differentiation
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
44XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)