KLRMMEi001-A
General#
Cell Line |
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| hPSCreg Name | KLRMMEi001-A |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 10th August 2018 |
| User feedback | |
Provider |
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| Generator | Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University. (KLRMME) |
| Owner | Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University. (KLRMME) |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA104626790 |
| Cellosaurus | CVCL_VE03 |
General Information |
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| Publications | |
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Is clinical information available? | yes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unkown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA104626791 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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| Source cell type |
olfactory epitheliumA sensory epithelium inside the nasal cavity that is responsible for detecting odors[WP].
Synonyms
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| Source cell origin |
urineExcretion that is the output of a kidney
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| Age of donor (at collection) | 25-29 |
| Collected in | 2017 |
| Passage number reprogrammed | 1 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
Immunostaining, PCR, RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method | Mechanically |
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
In vitro three germ layer differentiation immunofluorescence staining
Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
44XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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