GENEA021
GENEAe021-A
General
Cell Line |
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| hPSCreg name | GENEAe021-A |
| Cite as: | GENEAe021-A (RRID:CVCL_9030) |
| Alternative name(s) |
GENEA021
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
GENEAe016-A (GENEA022) |
| Last update | 3rd December 2019 |
| User feedback | |
Provider |
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| Generator |
Genea (GENEA)
Contact:
Biocells |
| Owner | Biocells |
| Distributors | |
| Derivation country | Australia |
External Databases |
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| BioSamples | SAMEA104130950 |
| Cellosaurus | CVCL_9030 |
| Wikidata | Q54835584 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
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| BioSamples | SAMEA104236910 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | two remaining files will be sent separtely as can only add one here. |
| Alternative consent approval number | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Genea HREC |
| Approval number | Meeting 13/9/2005 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Genea HREC |
| Approval number | Meeting 13/9/2005 |
hESC Derivation
| Date of derivation | 2008-08-25 |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
Yes |
Culture Conditions
| Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-4 |
Yes |
| Pluripotency Score | Novelty Score | |
| 21.85 | 1.42 |
Teratoma positive, Alkaline Phosphatase stain positive.
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
47XY, +21
Passage number: 44
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Other Genotyping (Cell Line) |
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