GENEAe016-A

GENEA022

General#

Cell Line

hPSCreg Name GENEAe016-A
Alternative name(s)
GENEA022
Cell line type Human embryonic stem cell (hESC)
Last update 20th April 2017
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Provider

Generator Genea (GENEA)
Contact:
Biocells
Owner Biocells
Distributors
Derivation country Australia

External Databases

BioSamples SAMEA104130951
Cellosaurus CVCL_9031

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

All cell lines of this donor's relatives
Has brother:
Has brother:

External Databases (Donor)

BioSamples SAMEA104130952

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2008-8-18
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions#

Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
FGF 50ng/mL %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score
42.95 1.23
Teratoma positive, Alkaline Phosphatase stain positive.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY no abnormalities detected

Other Genotyping (Cell Line)