GENEAe017-A

GENEA023

General#

Cell Line

hPSCreg Name GENEAe017-A
Alternative name(s)
GENEA023
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Genea (GENEA)
Contact:
Biocells
Owner Biocells
Derivation country Australia

External Databases

BioSamples SAMEA104236911
Cellosaurus CVCL_9032

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Donor Relations

All cell lines of this donor's relatives
Has brother:

External Databases (Donor)

BioSamples SAMEA104236912

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent other two documents will be sent separately as I can only add one at a time here.
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Genea HREC
Approval number Meeting 13/9/2005

hESC Derivation#

Date of derivation 2008-08-18
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions#

Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
FGF 50ng/mL %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
Pluripotency Score Novelty Score
42.76 1.23
Teratoma positive, Alkaline Phosphatase stain positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY male

Other Genotyping (Cell Line)