GENEAe017-A

GENEA023

General#

Cell Line

hPSCreg Name GENEAe017-A
Alternative name(s)
GENEA023
Cell line type Human embryonic stem cell (hESC)
Last update 20th April 2017
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Provider

Generator Genea (GENEA)
Contact:
Biocells
Owner Biocells
Derivation country Australia

External Databases

BioSamples SAMEA104236911
Cellosaurus CVCL_9032

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Donor Relations

All cell lines of this donor's relatives
Has brother:

External Databases (Donor)

BioSamples SAMEA104236912

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2008-08-18
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions#

Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
FGF 50ng/mL %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
Pluripotency Score Novelty Score
42.76 1.23
Teratoma positive, Alkaline Phosphatase stain positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY male

Other Genotyping (Cell Line)