Derivation of human embryonic stem cell line Genea022
Summary
The Genea022 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea022 was demonstrated with 84% of cells expressed Nanog, 98% Oct4, 55% Tra1-60 and 97% SSEA4, gave a Pluritest Pluripotency score of 42.95, Novelty of 1.23, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination. Copyright © 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.
| Authors | Dumevska B, Bosman A, McKernan R, Schmidt U, Peura T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2016 Mar;16(2):472-5 |
| PubMed | 27346017 |
| DOI | 10.1016/j.scr.2016.02.011 |