Derivation of Trisomy 21 affected human embryonic stem cell line Genea021
Summary
The Genea021 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Trisomy 21, indicative of Down Syndrome. Following ICM outgrowth on inactivated human feeders, CGH and STR analyses demonstrated a 47, XY, +21 karyotype and male allele pattern. The hESC line had pluripotent cell morphology, 71% of cells expressed Nanog, 84% Oct4, 23% Tra1-60 and 95% SSEA4, gave a Pluritest Pluripotency score of 21.85, Novelty of 1.42, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination. Copyright © 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.
| Authors | Dumevska B, Bosman A, McKernan R, Main H, Schmidt U, Peura T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2016 Mar;16(2):401-4 |
| PubMed | 27346003 |
| DOI | 10.1016/j.scr.2016.02.010 |