Generation of human induced pluripotent stem cells (CHULAi001-A) from peripheral blood mononuclear cells of a glucose-6-phosphate dehydrogenase (G6PD) deficient subject carrying the Viangchan mutation
Summary
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common genetic enzyme-defect disorder. In this study, CHULAi001-A was established from peripheral blood mononuclear cells of a G6PD-deficient subject who carried the Viangchan variant (871 G > A). Episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28, and shRNA against p53 were introduced into parental cells by electroporation and cultured under feeder-free conditions to reprogram iPSCs. Embryonic pluripotency, in vitro differentiation capacity, and episomal vector integration of the established CHULAi001-A were verified. The CHULAi001-A iPSCs retained a normal karyotype. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Tongbaen M, Matsumura Y, Okita K, Leecharoenkiat K |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Dec;65:102953 |
| PubMed | 36334578 |
| DOI | 10.1016/j.scr.2022.102953 |