PIDi003-A

The cell line is not validated yet.

General

Cell Line

hPSCreg name PHAi003-A
Cite as:
PHAi003-A (RRID:CVCL_YM65)
Alternative name(s)
PIDi003-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
PHAi003-B
(PIDi003-B)
Donor diseases:
Primary immunodeficiency
Last update 29th November 2021
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Provider

Generator Pan-Hammarström laboratory (PHA)
Owner Pan-Hammarström laboratory (PHA)
Distributors
Derivation country Sweden

External Databases

BioSamples SAMEA6324717
Cellosaurus CVCL_YM65
Wikidata Q98128583

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity persian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Primary immunodeficiency (PID, MIM# 602840 )
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
Homozygous
CD70 c.250delT
Family history available at 10.1084/jem.20160849
Is the medical history available upon request? yes, available at 10.1084/jem.20160849
Is clinical information available? available at 10.1084/jem.20160849

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
available at Arias-Fuenzalida et al., 2019 Stem Cell Research
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6324718

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. hassan.abolhassani@ki.se
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information hassan.abolhassani@ki.se
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regionala Etikprövningsnämnden Stockholm
Approval number 2014/946-31
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Regionala Etikprövningsnämnden Stockholm
Approval number 2014/946-31
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
CD184
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CD144
Yes
CD140b
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
SOX2
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX

Other Genotyping (Cell Line)