PIDi003-B

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name PHAi003-B
Alternative name(s)
PIDi003-B
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th November 2019
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Provider

Generator Pan-Hammarström laboratory (PHA)
Owner Pan-Hammarström laboratory (PHA)
Distributors
Derivation country Sweden

External Databases

BioSamples SAMEA6324719

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Ethnicity persian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Primary immunodeficiency (PID, MIM# 602840 )
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
Homozygous
CD70 c.250delT
Family history available at 10.1084/jem.20160849
Is the medical history available upon request? yes, available at 10.1084/jem.20160849
Is clinical information available? available at 10.1084/jem.20160849

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6324718

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: hassan.abolhassani@ki.se
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink hassan.abolhassani@ki.se
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regionala Etikprövningsnämnden Stockholm
Approval number 2014/946-31
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Regionala Etikprövningsnämnden Stockholm
Approval number 2014/946-31
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Fibroblasts

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
SOX17
Yes
CD184
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
CD144
Yes
CD140b
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
SOX2
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX

Other Genotyping (Cell Line)