HAD-C 100 Single Cell, Feeder-Free Bank (Seed Cell Bank, SCB)

General

Cell Line

hPSCreg name HADe007-B
Cite as:
HADe007-B (RRID:CVCL_B860)
Alternative name(s)
HAD-C 100 Single Cell, Feeder-Free Bank (Seed Cell Bank, SCB)
Cell line type Human embryonic stem cell (hESC)
Similar lines
Last update 14th April 2022
Notes This cell line is GMP-grade and xeno-free, single-cell and feeder-free.
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Provider

Generator Hadassah University Hospital (HAD)
Owner Hadassah University Hospital (HAD)
Distributors

External Databases

BioSamples SAMEA6560458
Cellosaurus CVCL_B860
NIHhESC NIHhESC-11-0123
Wikidata Q54872320

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: not allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:

There is a matching research-grade version of this cell-line available for interested parties. Ethical restrictions apply for this cell line. Inquire with us if interested.

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history No diseases
Is the medical history available upon request? Yes (brief summary of donors' medical history)
Is clinical information available? No health conditions

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6560651

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? Yes
Alternatives to consent Other information provided sep.
Alternative consent approval number NIHhESC-11-0123
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? IRB
Approval number 33-26-07.02
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Hadassah ESCRO Committee
Approval number N/A

hESC Derivation

Date of derivation 2008-09-01
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions

Feeder cells
No
Passage method Enzymatically
TrypLE
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
SOX17
Ont Id: OGG_3000064321
In vitro directed differentiation
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Brachyury Protein Antibody
Ont Id: NCIT_C113840
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Tubulin Beta 3 Class III
Ont Id: Orphanet_226013
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes