Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers


This study indicates that establishment of hES cells from frozen-thawed blastocysts minimizes the ethical problem associated with the use of human embryos in research and that the STO cell feeder layer can be used for the culture of hES cells.

Authors Park SP, Lee YJ, Lee KS, Ah Shin H, Cho HY, Chung KS, Kim EY, Lim JH
Journal Human reproduction (Oxford, England)
Publication Date 2004 Mar;19(3):676-84
PubMed 14998970
DOI 10.1093/humrep/deh102

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