Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers
Summary
This study indicates that establishment of hES cells from frozen-thawed blastocysts minimizes the ethical problem associated with the use of human embryos in research and that the STO cell feeder layer can be used for the culture of hES cells.
| Authors | Park SP, Lee YJ, Lee KS, Ah Shin H, Cho HY, Chung KS, Kim EY, Lim JH |
|---|---|
| Journal | Human reproduction (Oxford, England) |
| Publication Date | 2004 Mar;19(3):676-84 |
| PubMed | 14998970 |
| DOI | 10.1093/humrep/deh102 |