Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype
Summary
Here, we described the generation of human induced pluripotent stem cells (iPSC) from peripheral blood mononuclear cells (PBMCs) of a 87-year-old female patient with sporadic Alzheimer's disease (sAD) having APOE3 (ε3/ε3) genotype. iPSC line were generated from PBMCs with four factors of OCT4, SOX2, c-MYC and KLF4 using episomal system. The pluripotency of the iPSC line was assessed by embryoid body (EB) formation. Flow cytometry analyses revealed >97% cells positive for the pluripotency markers NANOG, OCT4 and SSEA4. Furthermore, the iPSC line displayed a normal karyotype (46, XX). The iPSC line may provide valuable tools for the study of sAD pathogenesis. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Zhang L, Ren Q, Liu X, Liu G, Meng S, Xiahou K, Zhang Y, Jiang N, Zhou W |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102282 |
| PubMed | 33799279 |
| DOI | 10.1016/j.scr.2021.102282 |