Generation of RUNX1c-eGFP induced pluripotent stem cell, MUSIi012-A-4, using CRISPR/Cas9
Summary
Runt-Related Transcription Factor 1c (RUNX1c) plays an important role in regulating the development of hematopoietic stem cells (HSC). Using CRISPR/Cas9 gene editing technology, we established a RUNX1c-eGFP reporter cell line from the MUSIi012-A cell line. The MUSIi012-A-4 cell line has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro and in vivo. This cell line serves as a valuable model to observe the expression of RUNX1c via eGFP tracking during human hematopoietic development. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Srisook P, Laowtammathron C, Lorthongpanich C, Klaihmon P, Terbto P, Waeteekul S, U-Pratya Y, Issaragrisil S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Mar;67:103035 |
| PubMed | 36753833 |
| DOI | 10.1016/j.scr.2023.103035 |