NY0001-01-ES-001
NYSCFe001-A
General
Cell Line |
|
| hPSCreg name | NYSCFe001-A |
| Cite as: | NYSCFe001-A (RRID:CVCL_LC26) |
| Alternative name(s) |
NY0001-01-ES-001
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 24th June 2021 |
| User feedback | |
Provider |
|
| Generator | New York Stem Cell Foundation Research Institute (NYSCF) |
| Owner | New York Stem Cell Foundation Research Institute (NYSCF) |
| Distributors | |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMEA104618138 |
| Cellosaurus | CVCL_LC26 |
| NIHhESC | NIHhESC-17-0389 |
| Wikidata | Q54931469 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Clinical use: not allowed
Commercial use: allowed
|
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | White |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA104618139 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-17-0389 |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Western Institutional Review Board |
| Approval number | Pro # 20101912 |
hESC Derivation
| Date of derivation | 2016-04-11 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
A
|
| Trophectoderm morphology |
a
|
| ZP removal technique | |
| Cell isolation | Laser |
| Cell seeding | Isolated ICM |
| Derived under xeno-free conditions? |
No |
| Derivation under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: DEMEM/F12/Glutamax (1x)
Main protein source: Freedom media (Life Technologies custom media #A14577SA) Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TRA 1-81 |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-4 |
Yes |
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
8
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
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