Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free conditions


The human embryonic stem cell line NYSCFe001-A was derived from a day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe001-A line, registered as NYSCF100 on the NIH registry, presents normal karyotype, is mycoplasma free, expresses all the pluripotency markers and has the potential to differentiate into all three germ layers in vitro. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Sevilla A, Forero E, Zimmer M, Martinez H, Reggio K, Paull D, Egli D, Noggle S
Journal Stem cell research
Publication Date 2018 May;29:99-102
PubMed 29631040
DOI 10.1016/j.scr.2018.03.017

Research Projects

Cell Lines