General

Cell Line

hPSCreg name CSSi011-A
Cite as:
CSSi011-A (RRID:CVCL_ZA85)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SAPi002-A
(ALS I, ALS I–FUS-R514S/wt, FUS-R514S/wt)
Donor diseases:
amyotrophic lateral sclerosis
UCSCi001-A
(SII-1802)
Donor diseases:
Amyotrophic Lateral Sclerosis
SAPi004-A
(ALS III, TDP43-A382T/A382T, ALS III-TDP43-A382T/A382T)
Donor diseases:
Amyotrophic lateral sclerosis
PFIZi013-A
(RCi215, RCFB59 C9)
Donor's gene variants:
TARDBP, TARDBP
Donor diseases:
Amyotrophic lateral sclerosis
HVRDi009-A
(29e SOD1 L144F, 29e)
Donor diseases:
obsolete_amyotrophic lateral sclerosis
NIMHi008-A
(NphyiALS1)
Donor's gene variants:
LDB3
Donor diseases:
Amyotrophic Lateral Sclerosis
UHi002-A
(HEL13.1, ALS50)
Donor diseases:
obsolete_amyotrophic lateral sclerosis
UHi003-A
(ALS75, HEL15.14)
Donor diseases:
obsolete_amyotrophic lateral sclerosis
UCLi004-A
(RCi173, RCFB60c6)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-B
(RCFB60c7, RCi177)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-C
(RCi172, RCFB60c2)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
SAPi003-A
(ALS II, FUS-R521C/wt, ALS II–FUS-R521C/wt)
Donor diseases:
Amyotrophic lateral sclerosis
CSSi012-A
Donor's gene variants:
TARDBP
Donor diseases:
Amyotrophic lateral sclerosis
ZZUi024-A
(ZZU-iPS-AD-APP-002)
Donor diseases:
Alzheimer's Disease
LCPHi001-A
Donor's gene variants:
GBA
Donor diseases:
obsolete_Parkinson's disease
ZZUi017-A
(ZZU-iPS-SCA6-001)
Donor diseases:
Spinocerebellar Ataxia Type 6
CSBZZUi001-A
(CSBZZU_PS1_001)
Donor diseases:
Alzheimer disease
LUMCi022-A
(115-1, LUMC0115iATAX01)
Donor diseases:
Spinocerebellar Ataxia Type 1
LUMCi022-B
(115-2, LUMC0115iATAX02)
Donor diseases:
Spinocerebellar Ataxia Type 1
LUMCi022-C
(115-7, LUMC0115iATAX07)
Donor diseases:
Spinocerebellar Ataxia Type 1
DANi004-A
(PRKN-004-C1)
Donor's gene variants:
PRKN
Donor diseases:
Parkinson Disease
DANi006-F
(GBA-006-C6)
Donor's gene variants:
GBA
Donor diseases:
Parkinson Disease
DANi011-A
(LRRK2-011-C1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
LCSBi009-A
(RHOT1_R272Q_clone1_PD)
Donor diseases:
Parkinson Disease
LCSBi010-A
(RHOT1_R450C_clone5_PD)
Donor diseases:
Parkinson Disease
CRICKi005-A
(iFCI004)
Donor diseases:
spinal muscular atrophy
CRICKi006-A
(iFCI005)
Donor diseases:
spinal muscular atrophy
AIBNi015-A
(SPG1-AU01C15)
Donor diseases:
hereditary spastic paraplegia 56
STBCi320-A
(SFC031-03-03)
Donor diseases:
Parkinson disease
LUMCi002-A
(113-6, LUMC0113iATAX06)
Donor diseases:
Spinocerebellar Ataxia Type 1
STBCi295-B
(SFC847-03-08)
Donor diseases:
Parkinson disease
LUMCi002-B
(113-7, LUMC0113iATAX07)
Donor diseases:
Spinocerebellar Ataxia Type 1
LCPHi003-A
Donor's gene variants:
LRP10
Donor diseases:
Parkinson Disease
Last update 20th May 2020
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Provider

Generator Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
Owner Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
Distributors
Derivation country Italy

External Databases

BioSamples SAMEA7044786
Cellosaurus CVCL_ZA85
Wikidata Q98125791

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • ALS
  • Lou Gehrig's disease
  • motor neuron disease, bulbar
Genetic variants

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA7044787

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Azienda Ospedaliera Universitaria “Maggiore della Carità" Novara Ethics Committee
Approval number CE54/17
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? PATSLA
Approval number CE54/17
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Nutristem XF Medium
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
iPSCs
Karyotyping method: G-Banding

Other Genotyping (Cell Line)