RCi215, RCFB59 C9

General#

Cell Line

hPSCreg Name PFIZi013-A
Alternative name(s)
RCi215, RCFB59 C9
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 2nd July 2019
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Provider

Generator Pfizer Limited - Pfizer (PFIZ)
Distributors

External Databases

BioSamples SAMEA4343623
Cellosaurus CVCL_IJ00
ECACC 66540322
EBiSC PFIZi013-A

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
amyotrophic lateral sclerosis
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Bulbar motor neuron disease
  • Lateral Scleroses, Amyotrophic
  • Amyotrophic Lateral Sclerosis, Guam Form
  • Amyotrophic lateral sclerosis, Parkinsonism/Dementia complex of Guam
  • Amyotrophic lateral sclerosis (disorder)
  • ALS (Amyotrophic Lateral Sclerosis)
  • LOU GEHRIG DIS
  • ALS
  • Gehrig's Disease
  • Dementia With Amyotrophic Lateral Sclerosis
  • Lou-Gehrigs Disease
  • Gehrigs Disease
  • AMYOTROPHIC SCLEROSIS
  • GEHRIGS DIS
  • ALS - Amyotrophic lateral sclerosis
  • Sclerosis, Amyotrophic Lateral
  • Guam Form of Amyotrophic Lateral Sclerosis
  • Disease, Lou-Gehrigs
  • Motor Neuron Disease, Amyotrophic Lateral Sclerosis
  • Lou Gehrigs Disease
  • Lou Gehrig's Disease
  • MOTOR NEURON DIS AMYOTROPHIC LATERAL SCLEROSIS
  • Amyotrophic Lateral Sclerosis With Dementia
  • Lou Gehrig Disease
  • Motor neuron disease, bulbar
  • Amyotrophic Lateral Sclerosis-Parkinsonism/dementia Complex 1
  • LOU GEHRIGS DIS
  • Gehrig Disease
  • Charcot disease
  • Lou Gehrig disease
  • amyotrophic lateral sclerosis
  • Lou Gehrig's disease
  • motor neuron disease, bulbar
show more synonyms
Genetic variants
TARDBP (target)
1p36.2
NM_007375.3:c.1144G>A
NP_031401.1:p.Ala382Thr
p.A382T mutation in TARDBP

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4343604

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink Clinical Department at UCL IoN
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Is there other documentation provided to the donor for consenting purposes? Yes
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? The National Hospital for Neurology and Neurosurgery & Institute of Neurology Joint REC
Approval number 09/H0716/64
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? The National Hospital for Neurology and Neurosurgery & Institute of Neurology Joint REC
Approval number 09/H0716/64
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Passage number reprogrammed 4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Unknown
GATA6
Unknown
CXCR4
Unknown
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
VIM
Unknown
DCN
Unknown
MIXL1
Unknown
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
NeuroD1
Yes
HES5
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 26

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuplodies were detected
Karyotyping method: BoBs

Other Genotyping (Cell Line)