19a

The cell line is not validated yet.

General

Cell Line

hPSCreg name HVRDi010-A
Cite as:
HVRDi010-A
Alternative name(s)
19a
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
PFIZi013-A
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TARDBP, TARDBP
Donor diseases:
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obsolete_amyotrophic lateral sclerosis
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LDB3
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Amyotrophic Lateral Sclerosis
UHi002-A
(HEL13.1, ALS50)
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obsolete_amyotrophic lateral sclerosis
UHi003-A
(ALS75, HEL15.14)
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obsolete_amyotrophic lateral sclerosis
UCLi004-A
(RCi173, RCFB60c6)
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C9orf72, C9orf72
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Frontotemporal dementia
UCLi004-B
(RCFB60c7, RCi177)
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C9orf72, C9orf72
Donor diseases:
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Frontotemporal dementia
UCLi004-C
(RCi172, RCFB60c2)
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C9orf72, C9orf72
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SAPi003-A
(ALS II, FUS-R521C/wt, ALS II–FUS-R521C/wt)
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Huntington disease
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HTT, HTT
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(HD8yrs)
Donor diseases:
Juvenile Huntington disease
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(KY03AP)
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Parkinson Disease
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Machado-Joseph disease
EDi001-A
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APOE, APOE
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Alzheimer disease
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Alzheimer's Disease
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MAPT
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Frontotemporal dementia
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VCP
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CSSi004-A
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Huntington disease
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obsolete_multiple sclerosis
UKERi004-A
(UKERi88H-R1-001)
Donor diseases:
obsolete_Parkinson's disease
UKBi003-A
(iLB-MJD1-32m-r9, LB-32-r9)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Machado-Joseph disease
UKBi008-A
(iLB-MJD4-34m-r1, LB-34-1)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Machado-Joseph disease
Last update 11th May 2021
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Provider

Generator Harvard University (HVRD)

External Databases

BioSamples SAMEA8927126

General Information

Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Bulbar motor neuron disease
  • Lateral Scleroses, Amyotrophic
  • Amyotrophic Lateral Sclerosis, Guam Form
  • Amyotrophic lateral sclerosis, Parkinsonism/Dementia complex of Guam
  • Amyotrophic lateral sclerosis (disorder)
  • ALS (Amyotrophic Lateral Sclerosis)
  • LOU GEHRIG DIS
  • ALS
  • Gehrig's Disease
  • Dementia With Amyotrophic Lateral Sclerosis
  • Lou-Gehrigs Disease
  • Gehrigs Disease
  • AMYOTROPHIC SCLEROSIS
  • GEHRIGS DIS
  • ALS - Amyotrophic lateral sclerosis
  • Sclerosis, Amyotrophic Lateral
  • Guam Form of Amyotrophic Lateral Sclerosis
  • Disease, Lou-Gehrigs
  • Motor Neuron Disease, Amyotrophic Lateral Sclerosis
  • Lou Gehrigs Disease
  • MOTOR NEURON DIS AMYOTROPHIC LATERAL SCLEROSIS
  • Amyotrophic Lateral Sclerosis With Dementia
  • Amyotrophic Lateral Sclerosis-Parkinsonism/dementia Complex 1
  • LOU GEHRIGS DIS
  • Gehrig Disease
  • Charcot disease
  • Lou Gehrig's Disease
  • Lou Gehrig Disease
  • Motor neuron disease, bulbar
show more synonyms

External Databases (Donor)

BioSamples SAMEA5176973

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Harvard University Area IRB
Approval number F13944
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Harvard University Area IRB
Approval number F13944
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: see MTA
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Any skin fibroblast that is part of some dermis.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex or mTeSR
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-81
Yes
Self-renewal
Unknown
Endoderm
Unknown
Mesoderm
Unknown
Ectoderm score
Positive

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX, Normal Human Female

Other Genotyping (Cell Line)