ALS II, FUS-R521C/wt, ALS II–FUS-R521C/wt

General#

Cell Line

hPSCreg Name SAPi003-A
Alternative name(s)
ALS II, FUS-R521C/wt, ALS II–FUS-R521C/wt
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th November 2019
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Provider

Generator Sapienza University of Rome (SAP)
Owner Sapienza University of Rome (SAP)
Distributors
Derivation country Italy

External Databases

BioSamples SAMEA6358471

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Amyotrophic lateral sclerosis
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Lou Gehrig disease
  • Charcot disease
  • ALS
Genetic variants

External Databases (Donor)

BioSamples SAMEA6358472

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Professor Adriano Chio, ‘Rita Levi Montalcini’ Department of Neuroscience, Torino I-10126, Italy; achio@usa.net
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink Professor Adriano Chio, ‘Rita Levi Montalcini’ Department of Neuroscience, Torino I-10126, Italy; achio@usa.net
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Comitato etico dell'azienda sanitaria ospedaliera
Approval number 0008457
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell line name fibroblasts
Source cell type
Fibroblasts

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Nutristem-XF/FF
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Cell Of Skeletal Muscle
Ont Id: CL_0000188
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Neuron
Ont Id: CL_0000540
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)