The cell line is not validated yet.

General#

Cell Line

hPSCreg Name HVRDi008-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th October 2018
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Provider

Generator Harvard University (HVRD)

External Databases

BioSamples SAMEA5176971

General Information

Publications
Projects
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
amyotrophic lateral sclerosis
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Bulbar motor neuron disease
  • Lateral Scleroses, Amyotrophic
  • Amyotrophic Lateral Sclerosis, Guam Form
  • Amyotrophic lateral sclerosis, Parkinsonism/Dementia complex of Guam
  • Amyotrophic lateral sclerosis (disorder)
  • ALS (Amyotrophic Lateral Sclerosis)
  • LOU GEHRIG DIS
  • ALS
  • Gehrig's Disease
  • Dementia With Amyotrophic Lateral Sclerosis
  • Lou-Gehrigs Disease
  • Gehrigs Disease
  • AMYOTROPHIC SCLEROSIS
  • GEHRIGS DIS
  • ALS - Amyotrophic lateral sclerosis
  • Sclerosis, Amyotrophic Lateral
  • Guam Form of Amyotrophic Lateral Sclerosis
  • Disease, Lou-Gehrigs
  • Motor Neuron Disease, Amyotrophic Lateral Sclerosis
  • Lou Gehrigs Disease
  • Lou Gehrig's Disease
  • MOTOR NEURON DIS AMYOTROPHIC LATERAL SCLEROSIS
  • Amyotrophic Lateral Sclerosis With Dementia
  • Lou Gehrig Disease
  • Motor neuron disease, bulbar
  • Amyotrophic Lateral Sclerosis-Parkinsonism/dementia Complex 1
  • LOU GEHRIGS DIS
  • Gehrig Disease
  • Charcot disease
show more synonyms

External Databases (Donor)

BioSamples SAMEA5176971

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex or mTeSR
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
SMA alpha
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
TUJ1
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX, Normal Human Female
Karyotyping method: G-Banding

Other Genotyping (Cell Line)