29e SOD1 L144F, 29e

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name HVRDi009-A
Alternative name(s)
29e SOD1 L144F, 29e
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th June 2019
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Provider

Generator Harvard University (HVRD)

External Databases

BioSamples SAMEA5176972
Cellosaurus CVCL_9002

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
amyotrophic lateral sclerosis
Synonyms
  • Bulbar motor neuron disease
  • Lateral Scleroses, Amyotrophic
  • Amyotrophic Lateral Sclerosis, Guam Form
  • Amyotrophic lateral sclerosis, Parkinsonism/Dementia complex of Guam
  • Amyotrophic lateral sclerosis (disorder)
  • ALS (Amyotrophic Lateral Sclerosis)
  • LOU GEHRIG DIS
  • ALS
  • Gehrig's Disease
  • Dementia With Amyotrophic Lateral Sclerosis
  • Lou-Gehrigs Disease
  • Gehrigs Disease
  • AMYOTROPHIC SCLEROSIS
  • GEHRIGS DIS
  • ALS - Amyotrophic lateral sclerosis
  • Sclerosis, Amyotrophic Lateral
  • Guam Form of Amyotrophic Lateral Sclerosis
  • Disease, Lou-Gehrigs
  • Motor Neuron Disease, Amyotrophic Lateral Sclerosis
  • Lou Gehrigs Disease
  • Lou Gehrig's Disease
  • MOTOR NEURON DIS AMYOTROPHIC LATERAL SCLEROSIS
  • Amyotrophic Lateral Sclerosis With Dementia
  • Lou Gehrig Disease
  • Motor neuron disease, bulbar
  • Amyotrophic Lateral Sclerosis-Parkinsonism/dementia Complex 1
  • LOU GEHRIGS DIS
  • Gehrig Disease
  • Charcot disease
  • Lou Gehrig disease
  • amyotrophic lateral sclerosis
  • Lou Gehrig's disease
  • motor neuron disease, bulbar
show more synonyms

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5176972

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Policy for access to genetic information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Harvard University Area IRB
Approval number F13944
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Harvard University Area IRB
Approval number F13944
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: see MTA
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex or mTeSR
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SMA alpha
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUJ1
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX, Normal Human Female

Other Genotyping (Cell Line)