CBIGi022-A-1

SOD1 I114T correction (CBIGi022-A), IPSC0029

The cell line is not validated yet.

General

Cell Line

hPSCreg name CBIGi022-A-1
Cite as:
CBIGi022-A-1
Alternative name(s)
SOD1 I114T correction (CBIGi022-A), IPSC0029
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 28th March 2025
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Provider

Generator Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG)
Distributors
Derivation country Canada

External Databases

BioSamples SAMEA117959514

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Amyotrophic Lateral Sclerosis
  • ALS
  • Lou Gehrig Disease
  • Amyotrophic lateral sclerosis
show more synonyms
Genetic variants
SOD1 (target)

External Databases (Donor)

BioSamples SAMEA117959513

Ethics

Also have a look at the ethics information for the parental line CBIGi022-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line CBIGi022-A.
Passage number reprogrammed P11

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal 46, XX
Passage number: P11
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Amyotrophic Lateral Sclerosis
  • ALS
  • Lou Gehrig Disease
  • Amyotrophic lateral sclerosis
show more synonyms
Genetic modifications
SOD1 (target)
Isogenic modification
21q22.11
After CRISP/Cas9 editing, Sanger sequencing confirms that the wt codon ATT has replaced the mutant codon ACT, correcting the missense mutation I114T.
Repaired