CRICKi007-A

iFCI009

General

Cell Line

hPSCreg Name
CRICKi007-A
Alternative name(s)
iFCI009
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
PFIZi013-A
(RCi215, RCFB59 C9)
Donor's gene variants:
TARDBP, TARDBP
Donor diseases:
Amyotrophic lateral sclerosis
CHDIi001-A
(#1c8, CHDI-90002149)
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HTT, HTT
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Huntington disease
CHDIi003-A
(#3c1, CHDI-90002151)
Donor's gene variants:
HTT, HTT
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Huntington disease
CHDIi005-A
(#5c4, CHDI-90002153)
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HTT, HTT
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Huntington disease
CHDIi020-A
(#20c2, CHDI-90002168)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi022-A
(#22c1, CHDI-90002170)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi055-A
(#133c5, CHDI-90002203)
Donor's gene variants:
HTT, HTT
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Huntington disease
STBCi013-A
(SFC807-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-A
(SFC808-03-03)
Donor's gene variants:
PSEN1
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Alzheimer disease
STBCi024-A
(SFC831-03-01)
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SNCA
Donor diseases:
Parkinson disease
STBCi019-C
(SFC828-03-04)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi061-A
(SFC850-03-01)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
STBCi062-A
(SFC851-03-04)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
STBCi004-A
(SFC832-03-19)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi084-A
(SFC871-03-12)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi084-C
(SFC871-03-09)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
CHDIi010-A
(#10c2, CHDI-90002158)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi026-A
(#26c3, CHDI-90002174)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi029-A
(#29c4, CHDI-90002177)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi031-A
(#31c1, CHDI-90002179)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
UCLi004-B
(RCFB60c7, RCi177)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-C
(RCi172, RCFB60c2)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
WTSIi153-A
(HPSI0414i-puvg_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi156-A
(HPSI0414i-xugr_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi157-A
(HPSI0614i-eavo_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi162-A
(HPSI0914i-lopq_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi166-A
(HPSI0414i-wize_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi033-A
(HPSI1013i-aetc_1)
Donor diseases:
Bardet-Biedl syndrome
STBCi031-A
(SFC024-04-02)
Donor diseases:
migraine disorder
CHDIi002-A
(#2c3, CHDI-90002150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi006-A
(#6c7, CHDI-90002154)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
UOXFi002-B
(MK082-30)
Donor's gene variants:
GBA
Donor diseases:
Parkinson disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi023-B
(SFC829-03-04)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi320-A
(SFC031-03-03)
Donor diseases:
Parkinson disease
EDi008-B
(G51D-4, EDINi008-B, EDIi008-B, SAMEA3174606)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
STBCi065-A
(SFC853-03-03)
Donor diseases:
Alzheimer disease
STBCi005-A
(SFC833-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-A
(SFC855-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi085-A
(SFC866-03-06)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi083-B
(SFC830-04-08)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi088-A
(SFC872-03-05)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
Last update 12th December 2022
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Provider

Generator The Francis Crick Institute Limited (CRICK)
Owner The Francis Crick Institute Limited (CRICK)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA112227670
Cellosaurus CVCL_C3R2
CLO CLO_0104314
Wikidata Q116048790

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
spinal muscular atrophy with lower extremity predominant

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA112227671

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink https://www.ucl.ac.uk/child-health/mrc-cnmd-biobank-london
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? The London – West London & GTAC Research Ethics Committee (formerly known as the Hammersmith, Queen Charlotte's and Chelsea Research Ethics Committee).
Approval number 08ND17
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Selection criteria for clones CHOSEN BY BEST MORPHOLOGY. MANUALLY PICKED
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Morphology pictures
FLOW CYTOMETRY
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
The KaryoStat™ assay allows for digital visualization of chromosome aberrations with a resolution similar to g-banding karyotyping by relying on 150k SNP probes across the human genome. The size of structural aberration that can be detected is > 2 Mb for chromosomal gains and > 1 Mb for chromosomal losses. The assay enables the detection of aneuploidies, submicroscopic aberrations, and mosaic events.

Other Genotyping (Cell Line)