A-257s2
UKBi014-A
General
Cell Line |
|
hPSCreg Name | UKBi014-A |
Alternative name(s) |
A-257s2
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 23rd March 2021 |
User feedback | |
Provider |
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Generator | Universitätsklinikum Bonn (UKB) |
Owner | ETH Zurich |
Distributors | |
Derivation country | Germany |
External Databases |
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BioSamples | SAMEA5782238 |
Cellosaurus | CVCL_YT14 |
CLO | CLO_0103015 |
Wikidata | Q98133955 |
General Information |
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* Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA5781289 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
Provide contact information of the holder of the original Donor Information Sheet: | Hans.vanBokhoven@radboudumc.nl |
Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
If you do not hold the Donor Consent Form, do you know who does? | No |
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | |
Has the donor agreed to be re-contacted? | Unknown |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
How may genetic information associated with the cell line be accessed? | Controlled Access |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Human Research Commission of Radboud UMC |
Approval number | 2015‐1543 |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
Name of accrediting authority involved? | Ethik Kommission Nordwest und Zentralschweiz (EKNZ) |
Approval number | 2019-00726 |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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Source cell type |
FibroblastSynonyms
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Differentiation Potency
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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