hiPSC NP0038
HDZi003-A
General
Cell Line |
|
| hPSCreg name | HDZi003-A |
| Cite as: | HDZi003-A (RRID:CVCL_D0QG) |
| Alternative name(s) |
hiPSC NP0038
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
HDZi001-A (hiPSC NP0039) Donor's gene variants: TMEM43 Donor diseases: arrhythmogenic right ventricular dysplasia 5 HIHDNDi001-A (A30P-3, SNCA3, Tue_020_A) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: autosomal dominant Parkinson disease 1 HIHDNDi001-B (A30P-4, SNCA4, Tue_020_B) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: autosomal dominant Parkinson disease 1 RNRMUi005-A (EB-iPSC-d4) Donor diseases: Epidermolysis Bullosa Simplex recessive dystrophic epidermolysis bullosa |
| Last update | 17th November 2023 |
| User feedback | |
Provider |
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| Generator | Heart and Diabetes Center North Rhine Westphalia (HDZ) |
| Owner | Heart and Diabetes Center North Rhine Westphalia (HDZ) |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA114251188 |
| Cellosaurus | CVCL_D0QG |
| Wikidata | Q123031574 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclones | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
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External Databases (Donor) |
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| BioSamples | SAMEA114251273 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | Torsten Bloch Rasmussen, MD; Aarhus Universiteshospital, Skejby, Hjertemedicinsk Afdeling B, Brendstrupgardsvej 100, 8200 Aarhus N |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | Torsten Bloch Rasmussen, MD; Aarhus Universiteshospital, Skejby, Hjertemedicinsk Afdeling B, Brendstrupgardsvej 100, 8200 Aarhus N |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethic committee of the Ruhr university Bochum in Bad Oeynhausen |
| Approval number | 41/2008 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Ethic committee of the Ruhr university Bochum in Bad Oeynhausen |
| Approval number | 41/2008 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Synonyms
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| Source cell origin |
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| Medium |
Other medium:
Base medium: StemMACS™ iPS-Brew XF
Main protein source: StemMACS™ iPS Brew XF, 50x Supplement Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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