hiPSC NP0038

General

Cell Line

hPSCreg name HDZi003-A
Cite as:
HDZi003-A (RRID:CVCL_D0QG)
Alternative name(s)
hiPSC NP0038
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
HDZi003-A-1
Donor's gene variants:
TMEM43
Donor diseases:
arrhythmogenic right ventricular dysplasia 5
HDZi001-A
(hiPSC NP0039)
Donor's gene variants:
TMEM43
Donor diseases:
arrhythmogenic right ventricular dysplasia 5
LUMCi002-A
(113-6, LUMC0113iATAX06)
Donor diseases:
Spinocerebellar Ataxia Type 1
LUMCi002-B
(113-7, LUMC0113iATAX07)
Donor diseases:
Spinocerebellar Ataxia Type 1
LUMCi002-C
(113-8, LUMC0113iATAX08)
Donor diseases:
Spinocerebellar Ataxia Type 1
ZJUi010-A
(ZJULLi002-A, 161103SPYLQTFSDC12)
Donor diseases:
Long QT Syndrome
IDIBGIi003-A
(​RB20235)
Donor diseases:
Brugada syndrome
FRIMOi004-A
(STGD2_ FiPS4F1.7)
Donor diseases:
Stargardt Disease
HIHDNDi001-A
(A30P-3, SNCA3, Tue_020_A)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
autosomal dominant Parkinson disease 1
HIHDNDi001-B
(A30P-4, SNCA4, Tue_020_B)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
autosomal dominant Parkinson disease 1
ZZUi026-A
(ZZU-iPS-SCA3-003)
Donor diseases:
Spinocerebellar Ataxia Type 3
CBRCULi016-A
(SCN5A p.R219H-14C)
Donor's gene variants:
SCN5A
Donor diseases:
dilated cardiomyopathy
VUi011-A
(SCZ 3.5)
Donor diseases:
Schizophrenia
ZZUi030-A
(ZZU-iPS-SPG7-001)
Donor diseases:
Spastic paraplegia type 7
DHMi004-A
(HOS_1460)
Donor diseases:
Holt-Oram Syndrome
UTSWi001-A
(FA1)
Donor diseases:
Friedreich Ataxia
UPITTi004-A
(CN090 C5A5J2)
Donor diseases:
Sickle cell anemia
UPITTi004-B
(CN090 C1B5B5)
Donor diseases:
Sickle cell anemia
MRIi017-A
(TRPM4)
Donor diseases:
Brugada syndrome
RMCGENi020-A
(IPS15-00004)
Donor diseases:
Stargardt Disease
RMCGENi021-A
(IPS22-00087)
Donor diseases:
Stargardt Disease
SCTCi017-A
(IPS15-00006)
Donor diseases:
Stargardt disease
SCTCi018-A
(IPS15-00007)
Donor diseases:
Stargardt disease
IDIBGIi002-A
(RB20234)
Donor diseases:
Brugada syndrome
IDIBGIi004-A
(RB20236)
Donor diseases:
Brugada syndrome
ZZUi017-A
(ZZU-iPS-SCA6-001)
Donor diseases:
Spinocerebellar Ataxia Type 6
UMCGi003-A
(PPCM RP1 c1)
Donor diseases:
peripartum cardiomyopathy
UMCGi003-B
(PPCM RP1 c3)
Donor diseases:
peripartum cardiomyopathy
UMCGi005-A
(PPCM RP2 c2)
Donor diseases:
peripartum cardiomyopathy
UMCGi005-B
(PPCM RP2 c9)
Donor diseases:
peripartum cardiomyopathy
FRIMOi003-A
(STGD1_ FiPS4F1.5)
Donor diseases:
Stargardt Disease
LUMCi022-A
(115-1, LUMC0115iATAX01)
Donor diseases:
Spinocerebellar Ataxia Type 1
LUMCi022-B
(115-2, LUMC0115iATAX02)
Donor diseases:
Spinocerebellar Ataxia Type 1
LUMCi022-C
(115-7, LUMC0115iATAX07)
Donor diseases:
Spinocerebellar Ataxia Type 1
Last update 17th November 2023
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Provider

Generator Heart and Diabetes Center North Rhine Westphalia (HDZ)
Owner Heart and Diabetes Center North Rhine Westphalia (HDZ)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA114251188
Cellosaurus CVCL_D0QG
Wikidata Q123031574

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • ARVC5
  • ARVD5
  • TMEM43 arrhythmogenic right ventricular cardiomyopathy
  • arrhythmogenic right ventricular cardiomyopathy 5
  • arrhythmogenic right ventricular cardiomyopathy caused by mutation in TMEM43
  • arrhythmogenic right ventricular dysplasia 5
  • arrhythmogenic right ventricular dysplasia type 5
  • arrhythmogenic right ventricular dysplasia, familial, type 5
  • familial arrhythmogenic right ventricular dysplasia 5
  • arrhythmogenic right ventricular dysplasia, familial, 5
show more synonyms
Genetic variants
TMEM43 (target)
Ser358Leu
Heterozygous

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing

External Databases (Donor)

BioSamples SAMEA114251273

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Torsten Bloch Rasmussen, MD; Aarhus Universiteshospital, Skejby, Hjertemedicinsk Afdeling B, Brendstrupgardsvej 100, 8200 Aarhus N
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Torsten Bloch Rasmussen, MD; Aarhus Universiteshospital, Skejby, Hjertemedicinsk Afdeling B, Brendstrupgardsvej 100, 8200 Aarhus N
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethic committee of the Ruhr university Bochum in Bad Oeynhausen
Approval number 41/2008
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethic committee of the Ruhr university Bochum in Bad Oeynhausen
Approval number 41/2008
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Source cell origin
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF
Main protein source: StemMACS™ iPS Brew XF, 50x Supplement
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)