hiPSC NP0039

General#

Cell Line

hPSCreg Name HDZi001-A
Alternative name(s)
hiPSC NP0039
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th July 2020
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Provider

Generator Heart and Diabetes Center North Rhine Westphalia (HDZ)
Owner Heart and Diabetes Center North Rhine Westphalia (HDZ)

External Databases

BioSamples SAMEA7001542
CLO CLO_0102539

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Ethnicity European

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
arrhythmogenic right ventricular dysplasia 5
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • familial arrhythmogenic right ventricular dysplasia 5
  • arrhythmogenic right ventricular cardiomyopathy 5
  • ARVD5
  • ARVC5
show more synonyms
Genetic variants
TMEM43 (target)
Please explain briefly the supporting evidence

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Array CGH

External Databases (Donor)

BioSamples SAMEA7001543

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Torsten Bloch Rasmussen, MD; Aarhus Universiteshospital, Skejby, Hjertemedicinsk Afdeling B, Brendstrupgardsvej 100, 8200 Aarhus N
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink Torsten Bloch Rasmussen, MD; Aarhus Universiteshospital, Skejby, Hjertemedicinsk Afdeling B, Brendstrupgardsvej 100, 8200 Aarhus N
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethic committee of the Ruhr university Bochum in Bad Oeynhausen
Approval number 41/2008
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethic committee of the Ruhr university Bochum in Bad Oeynhausen
Approval number 41/2008
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
dermal fibroblast
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Collagenase
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Desmin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Nestin
Unknown

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
stable
Karyotyping method: Array CGH

Other Genotyping (Cell Line)