Joub07 cl1, COR11-NG708

General#

Cell Line

hPSCreg Name CSSi007-A
Alternative name(s)
Joub07 cl1, COR11-NG708
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 17th January 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
Owner Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
Derivation country Italy

External Databases

BioSamples SAMEA5359104

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Joubert syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Joubert-Boltshauser syndrome
  • Pure Joubert syndrome
  • Joubert syndrome type A
  • Classic Joubert syndrome
  • Cerebelloparenchymal disorder IV
  • CPD IV
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5359105

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name dermal fibroblasts
Source cell type
dermal fibroblast
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.
Age of donor (at collection) 25-29
Collected in 2014

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology, immunofluorescent analysis, qRT-PCR
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Nutristem
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyogram
Karyotyping method: G-Banding

Other Genotyping (Cell Line)