Generation of a TMEM43 knockout human induced pluripotent stem cell line (HDZi003-A-1) using CRISPR/Cas9
Summary
TMEM43 (LUMA) is a ubiquitously expressed protein with unknown function. The protein is phylogenetically highly conserved and also found in Drosophila melanogaster (Klinke et al., 2022). TMEM43-p.S358L is a rare, fully penetrant mutation that leads to arrhythmogenic right ventricular cardiomyopathy type 5 (ARVC5). To understand the function of the ARVC5-associated mutation it is first important to understand the function of the TMEM43 protein. Therefore, a TMEM43 knockout induced pluripotent stem cell (iPSC) line was generated using the CRISPR/Cas9 genome editing system. The resulting cell line had a deficiency of TMEM43 and showed normal morphology and a stable karyotype. The colonies were positive for pluripotency markers and could be differentiated into the three germ layers. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ratnavadivel S, Dammeier J, Gaertner A, de Toledo MAS, Zenke M, Gummert J, Bloch Rasmussen T, Klinke N, Jürgens K, Meyer H, Paululat A, Milting H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103354 |
| PubMed | 38430734 |
| DOI | 10.1016/j.scr.2024.103354 |