LQTS006C8, LQT006C8

General#

Cell Line

hPSCreg Name NUIGi007-B
Alternative name(s)
LQTS006C8, LQT006C8
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator National University of Ireland Galway (NUIG)
Derivation country Ireland

External Databases

BioSamples SAMEA5912496
Cellosaurus CVCL_WS94

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 40-44
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Long QT Syndrome 1 (primary disease)
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Romano-Ward Syndrome
  • Long QT Syndrome 1
  • LQT1

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5912495

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Policy for access to genetic information No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
dermal fibroblast
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.
Age of donor (at collection) 40-44
Collected in 2016

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No gross chromosomal alterations detected
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)