Generation of eight human induced pluripotent stem cell (iPSC) lines from familial Long QT Syndrome type 1 (LQT1) patients carrying KCNQ1 c.1697C>A mutation (NUIGi005-A, NUIGi005-B, NUIGi005-C, NUIGi006-A, NUIGi006-B, NUIGi006-C, NUIGi007-A, and NUIGi007-B)
Summary
Long QT Syndrome type 1 (LQT1), an inherited cardiac ion channelopathy associated with arrhythmias and risk of sudden death, is caused by mutations in KCNQ1 encoding the α-subunit of Kv7.1, that affects the slow component of delayed rectifier K+ current (IKs) channel. In this study, the non-integrational Sendai reprogramming method was used to express four Yamanaka factors and to generate induced pluripotent stem cell (iPSC) lines carrying the KCNQ1 c.1697C>A (p.S566Y) mutation from familial LQT1 patients. The patient-specific iPSC lines harbouring the c.1697C>A mutation expressed pluripotency markers and had the capacity to differentiate into three germ layers. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ge N, Liu M, Krawczyk J, McInerney V, Galvin J, Shen S, O'Brien T, Prendiville T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Aug;39:101502 |
| PubMed | 31415974 |
| DOI | 10.1016/j.scr.2019.101502 |