UTA.00211.LQT1

General

Cell Line

hPSCreg name TAUi007-B
Cite as:
TAUi007-B (RRID:CVCL_RN18)
Alternative name(s)
UTA.00211.LQT1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
TAUi007-A
(UTA.00208.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
TAUi006-A
(UTA.00102.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
TAUi006-B
(UTA.00118.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
UKKi034-A
(NP0079-A, NP0079-7B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-B
(NP0079-B, NP0079-15B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-C
(NP0079-C, NP0079-16H)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
LUMCi039-A
(LQT1-1781G/A hiPSC, LUMC0021iKCNQ-30)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Long QT Syndrome 1
UKKi029-A
(NP0077-6D)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi029-B
(NP0077-16E)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi029-C
(NP0077-18C)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-B
(NP0078-12)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-C
(NP0078-13)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-A
(NP0078-10)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
NUIGi005-A
(LQTS004C7, LQT004C7)
Donor diseases:
Long QT Syndrome 1
NUIGi005-B
(LQTS004C9, LQT004C9)
Donor diseases:
Long QT Syndrome 1
NUIGi005-C
(LQTS004Cx, LQT004Cx)
Donor diseases:
Long QT Syndrome 1
NUIGi008-A
(LQTS007C1)
Donor diseases:
Long QT Syndrome 1
NUIGi008-B
(LQTS007C2)
Donor diseases:
Long QT Syndrome 1
NUIGi008-C
(LQTS007Cx)
Donor diseases:
Long QT Syndrome 1
NUIGi009-A
(LQTS008C1)
Donor diseases:
Long QT Syndrome 1
NUIGi009-B
(LQTS008C6)
Donor diseases:
Long QT Syndrome 1
NUIGi009-C
(LQTS008Cx)
Donor diseases:
Long QT Syndrome 1
NUIGi011-A
(LQTS010C3)
Donor diseases:
Long QT Syndrome 1
NUIGi011-B
(LQTS010C4)
Donor diseases:
Long QT Syndrome 1
NUIGi011-C
(LQTS010Cx)
Donor diseases:
Long QT Syndrome 1
NUIGi006-A
(LQTS005C2, LQT005C2)
Donor diseases:
Long QT Syndrome 1
NUIGi006-B
(LQTS005C4, LQT005C4)
Donor diseases:
Long QT Syndrome 1
NUIGi006-C
(LQTS005Cx, LQT005Cx)
Donor diseases:
Long QT Syndrome 1
NUIGi007-A
(LQTS006C6, LQT006C6)
Donor diseases:
Long QT Syndrome 1
NUIGi007-B
(LQTS006C8, LQT006C8)
Donor diseases:
Long QT Syndrome 1
NUIGi010-A
(LQTS009C2)
Donor diseases:
Long QT Syndrome 1
NUIGi010-B
(LQTS009C3)
Donor diseases:
Long QT Syndrome 1
NUIGi010-C
(LQTS009Cx)
Donor diseases:
Long QT Syndrome 1
BBANTWi007-A
(iPSC_BrS10_FB_C3)
Donor's gene variants:
SCN5A
Donor diseases:
Brugada syndrome 1
LUMCi027-A
(LUMC0153iPKP03)
Donor's gene variants:
PKP2
Donor diseases:
arrhythmogenic right ventricular dysplasia 9
ICANi001-A
(CDGEN1.16)
Donor diseases:
hypertrophic cardiomyopathy
BBANTWi006-A
(iPSC_BrS9_FB_C7)
Donor's gene variants:
SCN5A
Donor diseases:
Brugada syndrome 1
TAUi005-A
(UTA.14511.CPVT)
Donor's gene variants:
RYR2
Donor diseases:
Catecholaminergic polymorphic ventricular tachycardia
LUMCi030-A
(LUMC0110iALK04)
Donor diseases:
Hereditary Hemorrhagic Telangiectasia
LUMCi030-B
(LUMC0110iALK10)
Donor diseases:
Hereditary Hemorrhagic Telangiectasia
RCMGi008-A
(P9L1)
Donor diseases:
Cystic Fibrosis
UMCGi002-A
(CTP1-C1)
Donor diseases:
Cardiomyopathy
UMCGi002-B
(CTP1-C2)
Donor diseases:
Cardiomyopathy
RCMGi007-A
(P8L2)
Donor diseases:
Cystic Fibrosis
UMCGi002-C
(CTP1-C3)
Donor diseases:
Cardiomyopathy
RCMGi002-A
(P7L2)
Donor diseases:
Cystic Fibrosis
Last update 19th April 2022
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Provider

Generator Tampere University (TAU)
Derivation country Finland

External Databases

BioSamples SAMEA8549178
Cellosaurus CVCL_RN18
Wikidata Q54992203

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: ERC StG-project of Sara Liin
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Romano-Ward Syndrome
  • Long QT Syndrome 1
  • LQT1
Genetic variants
KCNQ1 – potassium voltage-gated channel subfamily Q member 1 (target)
KCNQ1FinA mutation G589D

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA8549179

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Pirkanmaa Hospital District
Approval number R08070
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Collected in 2008

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Gelatin
Feeder cells CF-1 MEF MITC
Cellfinder Ont Id: CELDA_00007731
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
FGF %
Glutamax
Pen/Strep
NEA
β-mercaptoethanol
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
KDR
Yes
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
nestin
Unknown
Protocol or reference

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)