Generation of a tdTomato-GAD67 reporter human epilepsia mutation induced pluripotent stem cell line, USTCi001-A-2, using CRISPR/Cas9 editing


Dravet syndrome is an epileptic encephalopathy largely due to haploinsufficiency of the voltage-gated sodium channel Nav1.1 that is expressed primarily in GABAergic neurons. In order to distinguish the different subtypes, we used gene editing to introduce tdTomato gene into the genome of iPSCs to label the GABAergic neurons in the differentiated neuronal networks. The gene-edited cell line demonstrates normal karyotype, expresses the main pluripotency markers, and shows the presence of differentiation into the three embryonic germ layers in teratomas. Copyright © 2020. Published by Elsevier B.V.

Authors Zhao H, He L, Huang H, Li S, Cheng N, Tang F, Han X, Lin Z, Huang R, Zhou P, Deng S, Huang J, Li Z
Journal Stem cell research
Publication Date 2020 Oct;48:102003
PubMed 32977294
DOI 10.1016/j.scr.2020.102003

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