General

Cell Line

hPSCreg name LCPHi002-A
Cite as:
LCPHi002-A (RRID:CVCL_B5G5)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
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EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
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EDi001-A-4
(AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6)
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SNCA, SNCA, SNCA, SNCA
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Parkinson disease
MPIi003-A-1
(IM2GC, L2-2GC)
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obsolete_Parkinson's disease
EDi001-B-1
(AST18-7A)
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EDi001-B-2
(AST18-7B)
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EDi001-B-3
(AST18-5D)
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EDi001-B-4
(AST18-6A)
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Parkinson disease
EDi001-A-5
(AST23-2KO-II8B)
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STBCi004-B-1
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LRRK2
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LCSBi010-A-2
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LCSBi012-A-1
(RHOT1_T610A_clone62.19.37_IsogenicControl)
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LCSBi008-A-1
(delP GC13, DJ-1-delP GC13)
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Parkinson Disease
SUSMi005-A-1
(SNCA3X 0KO C1, SNCA3X 0KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-2
(SNCA3X 1KO C2, SNCA3X 1KO C1)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-3
(SNCA3X 2KO C1, SNCA3X 2KO C2)
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obsolete_Parkinson's disease
SUSMi005-A-4
(SNCA3X 3KO C1, SNCA3X 3KO C2)
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obsolete_Parkinson's disease
SUSMi005-A-5
(SNCA3X 4KO C1, SNCA3X 4KO C2)
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LUEi017-A
(iPS-L-2122)
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(iPS-L-2124)
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ICGi015-B-1
(m6.7pCyto-17)
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Parkinson Disease
ICGi015-B-2
(m6.7pCyto-21)
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Parkinson Disease
ICGi015-B-3
(m6.7pCyto-24)
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ICGi034-A-1
(PD30-XBP-RFP-6, PD30-4-7-XBP-RFP-6)
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obsolete_Parkinson's disease
ICGi034-A-2
(PD30-4-7-XBP-RFP-51, PD30-XBP-RFP-51)
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obsolete_Parkinson's disease
LCSBi011-A-1
(RHOT1_T351A_clone25.2_IsogenicControl)
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LUEi014-A
(iPS-B-125)
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Parkinson Disease
LUEi015-A
(iPS-B-11)
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Parkinson Disease
LCPHi001-A
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GBA
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obsolete_Parkinson's disease
UNAMi002-A
(IFC-UNAM iPD02-S)
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UNAMi003-A
(IFC-UNAM iPD03-PINK1)
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Parkinson Disease
STBCi004-B
(SFC832-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-C
(SFC832-03-07)
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LRRK2
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Parkinson disease
PNUSCRi004-A
(GBA PD iPSC8)
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Parkinson Disease
CDIi015-A
(PPMI_3419, FCDI_11294)
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Parkinson Disease
SHEHDNi002-A
(KY03AP)
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LRRK2, DNAJC6
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Parkinson Disease
CDIi026-A
(FCDI_11307, PPMI_51625)
Donor diseases:
Parkinson Disease
CDIi027-A
(FCDI_11308, PPMI_3186)
Donor diseases:
Parkinson Disease
CDIi028-A
(PPMI_3664, FCDI_11309)
Donor diseases:
Parkinson Disease
CDIi038-A
(FCDI_11323, PPMI_53339)
Donor diseases:
Parkinson Disease
EDi001-A
(AST22, AST23, SAMEA3319992)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
UHi004-A
(HEL157.1)
Donor diseases:
obsolete_Parkinson's disease
CDIi040-A
(FCDI_11327, PPMI_3220)
Donor diseases:
Parkinson Disease
UHi004-B
(HEL157.3)
Donor diseases:
obsolete_Parkinson's disease
Last update 17th April 2022
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Provider

Generator The Institute for Tissue Engineering and Regenerative Medicine, Liaocheng People's Hospital (LCPH)
Owner The Institute for Tissue Engineering and Regenerative Medicine, Liaocheng People's Hospital (LCPH)
Derivation country China

External Databases

BioSamples SAMEA10558482
Cellosaurus CVCL_B5G5
Wikidata Q110433003

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 30-34
Ethnicity Han Chinese

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic variants
9q21.2
NM_033305.3: exon 37 c. 4282_4289delinsA
p. Ala1428fs
Heterozygous
Family history None
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA10768117

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. FABIN HAN, fhan2013@126.com
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Liaocheng People's Hospital
Approval number 201527
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Shinya Yamanaka

hIPSC Derivation

General

Source cell type
Source cell origin
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
  • Skin
  • Human Skin
  • SKIN
  • Integument
  • Skin, total
  • Skin, NOS
  • Skin structure (body structure)
  • entire skin
  • skin of body
  • skin organ
show more synonyms
Age of donor (at collection) 30-34
Collected in 2021
Passage number reprogrammed 4

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)