MUSCSDi001-A

iPSK3

General#

Cell Line

hPSCreg Name MUSCSDi001-A
Alternative name(s)
iPSK3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 26th May 2020
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Provider

Generator Steve's Duncan Lab (MUSCSD)

External Databases

BioSamples SAMEA7023730
CLO CLO_0100760

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7023731

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? No
Was the consent voluntarily given? No
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: De-identified fibroblasts were procured by ATCC in 1994 from a donor. No donor information is available. Please see attached letter from ATCC for further details.
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? Yes
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? MUSC Stem Cell Research Oversight Committee
Approval number MUSC SCRO #8
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell line name ATCC cell line CRL2097
Source cell type
skin fibroblast cell line
Source cell origin
skin fibroblast
Source cell line vendor ATCC

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells MEF
Passage method Enzymatically
Accutase
O2 Concentration 4 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/Ham's F-12
Main protein source:
Serum concentration: %
Supplements
KOSR 20 %
NEAA
Pen/Strep
bFGF 4 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
CD13
No
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro directed differentiation
Marker Expressed
HNF4A
Yes
AFP
Yes
Albumin
Yes
FoxA2
Yes
E-Cadherin
Yes
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
Gata4
Yes
TroponinT
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY (Normal)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)