Generation of human induced pluripotent stem cells by simple transient transfection of plasmid DNA encoding reprogramming factors
Summary
Simple transient transfection of plasmid DNA encoding reprogramming factors is sufficient to generate human iPS cells from primary fibroblasts that are free of exogenous DNA integrations. This approach is highly accessible and could expand the use of iPS cells in the study of human disease and development.
| Authors | Si-Tayeb K, Noto FK, Sepac A, Sedlic F, Bosnjak ZJ, Lough JW, Duncan SA |
|---|---|
| Journal | BMC developmental biology |
| Publication Date | 2010 Aug 3;10:81 |
| PubMed | 20682060 |
| PubMed Central | PMC2923111 |
| DOI | 10.1186/1471-213x-10-81 |