PCCB ∆14/∆29
MUSCSDi001-A-1
General
Cell Line |
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| hPSCreg name | MUSCSDi001-A-1 |
| Cite as: | MUSCSDi001-A-1 (RRID:CVCL_ZX59) |
| Alternative name(s) |
PCCB ∆14/∆29
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
MUSCSDi001-A-2 (PCCB ∆19/∆481) MUSCSDi001-A (iPSK3) |
| Last update | 29th September 2020 |
| User feedback | |
Provider |
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| Generator |
Steve's Duncan Lab (MUSCSD)
Contact:
Steve's Duncan Lab (MUSCSD) |
| Owner | Steve's Duncan Lab (MUSCSD) |
External Databases |
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| BioSamples | SAMEA7023718 |
| Cellosaurus | CVCL_ZX59 |
| Wikidata | Q102114568 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46 + XY (Normal)
Karyotyping method:
G-Banding
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7023731 |
Ethics
Also have a look at the ethics information for the parental line
MUSCSDi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Addgene |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
MUSCSDi001-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | eCadherin | ||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 4 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
mTeSR™ 1
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Normal 46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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