MUSCSDi001-A-1

PCCB ∆14/∆29

General

Cell Line

hPSCreg Name
MUSCSDi001-A-1
Alternative name(s)
PCCB ∆14/∆29
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 29th September 2020
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Provider

Generator Steve's Duncan Lab (MUSCSD)
Owner Steve's Duncan Lab (MUSCSD)

External Databases

BioSamples SAMEA7023718
CLO CLO_0102831
Cellosaurus CVCL_ZX59
Wikidata Q102114568

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46 + XY (Normal)
Karyotyping method: G-Banding

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7023731

Ethics

Also have a look at the ethics information for the parental line MUSCSDi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Addgene

hIPSC Derivation

General

The source cell information can be found in the parental cell line MUSCSDi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating eCadherin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 4 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Supplements
zbFGF 40 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
ASMA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal 46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
propionic acidemia
Genetic modifications
PCCB (target)
Gene knock-out
3q22.3
CRISPR-associated (CRISPR/Cas) System