UOHi001 with its alternative name as UOHi001SHANK3

General

Cell Line

hPSCreg name UOHi003-A
Cite as:
UOHi003-A (RRID:CVCL_C1XU)
Alternative name(s)
UOHi001 with its alternative name as UOHi001SHANK3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BCHi021-A-1
(HNDS0129-01#A CNC7(+/-))
Donor diseases:
Phelan-McDermid syndrome
BCHi021-A-2
(HNDS0129-01#A CC20(+/+))
Donor diseases:
Phelan-McDermid syndrome
BCHi021-A-3
(HNDS0129-01#A CC47(-/-))
Donor diseases:
Phelan-McDermid syndrome
IMGTi001-B
(iTAF5-32)
Donor diseases:
Phelan-McDermid syndrome
BCHi021-A
(HNDS0129-01#A)
Donor diseases:
Phelan-McDermid syndrome
IMGTi001-A
(iTAF5-29)
Donor diseases:
Phelan-McDermid syndrome
Last update 26th September 2022
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Provider

Generator University of Haifa (UOH)
Owner University of Haifa (UOH)
Distributors
Derivation country Israel

External Databases

Cellosaurus CVCL_C1XU
BioSamples SAMEA111396693
Wikidata Q114313208

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity Jewish

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Chromosome 22q13.3 Deletion Syndrome
  • Phelan-McDermid Syndrome
  • PHMDS
Disease associated phenotypes
  • Coelic disease
  • Insomnia
  • Epilepsy
Family history no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA110584747

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. ao2012@gmail.com
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information ao2012@gmail.com
Alternatives to consent are available? No
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? Yes
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? The Sheba Medical Center IRB
Approval number SMC-6158-19
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-4
Yes
SOX2
Yes
Marker Present Absent
mCpG
OCT4 X
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
Alpha-Fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
Smooth Muscle Actin
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Neurofilament
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
G-banding
Passage number: 5
Karyotyping method: G-Banding

Other Genotyping (Cell Line)