BCHi021-A-1

HNDS0129-01#A CNC7(+/-)

General

Cell Line

hPSCreg name BCHi021-A-1
Cite as:
BCHi021-A-1
Alternative name(s)
HNDS0129-01#A CNC7(+/-)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BCHi021-A-2
(HNDS0129-01#A CC20(+/+))
Donor diseases:
Phelan-McDermid syndrome
BCHi021-A-3
(HNDS0129-01#A CC47(-/-))
Donor diseases:
Phelan-McDermid syndrome
BCHi021-A
(HNDS0129-01#A)
Donor diseases:
Phelan-McDermid syndrome
UOHi003-A
(UOHi001 with its alternative name as UOHi001SHANK3)
Donor diseases:
Phelan-McDermid Syndrome
IMGTi001-B
(iTAF5-32)
Donor diseases:
Phelan-McDermid syndrome
IMGTi001-A
(iTAF5-29)
Donor diseases:
Phelan-McDermid syndrome
CEBe033-A-2
(SA001 Shank3 hetero1)
CEBe033-A-3
(SA001 Shank3 Hetero2)
CEBe033-A-4
(SA001 Shank3 Hetero3)
CEBe033-A-5
(SA001 Shank3 Homo1)
CEBe033-A-6
(SA001 Shank3 Homo2)
CEBe033-A-7
(SA001 Shank3 Homo3)
Last update 29th February 2024
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Children's Hospital (BCH)

External Databases

BioSamples SAMEA115361527

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • 22q13 deletion
  • Phelan McDermid syndrome
  • monosomy 22q13
  • monosomy type 22q13
  • 22q13.3 deletion syndrome
  • PHMDS
  • chromosome 22Q13.3 deletion syndrome
  • deletion 22q13.3 syndrome
  • telomeric 22Q13 monosomy syndrome
  • Phelan-McDermid syndrome
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

External Databases (Donor)

BioSamples SAMEA115361526

Ethics

Also have a look at the ethics information for the parental line BCHi021-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BCHi021-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Other medium:
Base medium: Stemflex
Main protein source: Bovine Serum Albumin (BSA)
Serum concentration: 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BCHi021-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY; no clonal abnormalities
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Chromosome 22q13.3 Deletion Syndrome
  • Phelan-McDermid Syndrome
  • PHMDS
Genetic modifications
SHANK3 (target)
Variant
cytoband location: 22q13
NM_033517.1: c.3679delG, p.A1227Pfs*57
Heterozygous