BCHi040-A-4

HNDS0130-01#B CC67(+/+)

General

Cell Line

hPSCreg name BCHi040-A-4
Cite as:
BCHi040-A-4
Alternative name(s)
HNDS0130-01#B CC67(+/+)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 31st May 2024
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Provider

Generator Children's Hospital (BCH)

External Databases

BioSamples SAMEA115580274

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Chromosome 22q13.3 Deletion Syndrome
  • Phelan-McDermid Syndrome
  • PHMDS

External Databases (Donor)

BioSamples SAMEA115527689

Ethics

Also have a look at the ethics information for the parental line BCHi040-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BCHi040-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Other medium:
Base medium: Stemflex
Main protein source: Bovine Serum Albumin (BSA)
Serum concentration: 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BCHi040-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX; no clonal abnormalities
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Chromosome 22q13.3 Deletion Syndrome
  • Phelan-McDermid Syndrome
  • PHMDS
Genetic modifications
SHANK3 (target)
Isogenic modification
cytoband location: 22q13
NM_033517.1: c.3679G; p.A1227
Homozygous
Repaired