niPSC HDF75, niPSC-75.1c2


Cell Line

hPSCreg name CSCIi002-A
Cite as:
Alternative name(s)
niPSC HDF75, niPSC-75.1c2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 11th April 2024
Notes Naive iPSC line expanded from a single colony generated by RNA reprogramming of human dermal fibroblasts [ThermoFisher Scientific product number C0135C, Adult Human Dermal Fibroblasts (HDFa), lot number 759506 (37 yo F)] as described by Bredenkamp et al. doi. 10.1016/j.stemcr.2019.10.009
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Generator Wellcome Trust - MRC Stem Cell Institute (CSCI)
Owner University of Cambridge (CAM)
Derivation country United Kingdom

External Databases

BioSamples SAMEA115466178

General Information

* Is the cell line readily obtainable for third parties?
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 35-39
Ethnicity Not available

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?

External Databases (Donor)

BioSamples SAMEA115462284


Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number The cells in this lot were derived from tissue obtained from accredited institutions. Consent was obtained by these institutions from the donor or the donor's legal next of kin, for use of the tissue and its derivatives for research purposes.
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? ReproCell
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation


Source cell line name Human dermal fibroblasts
Source cell type
Source cell origin
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
  • Skin
  • Human Skin
  • SKIN
  • Integument
  • Skin, total
  • Skin, NOS
  • Skin structure (body structure)
  • entire skin
  • skin of body
  • skin organ
show more synonyms
Source cell line lot number
Age of donor (at collection) 35-39
Collected in 2011
Source cell line vendor Thermo Fisher
Passage number reprogrammed 5

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)


Selection criteria for clones Single refractile domed colony picked and expanded
Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells Mouse embryo fibroblasts
Passage method Enzymatically
O2 Concentration 5 %
CO2 Concentration 7 %
Medium Other medium:
Base medium: DMEM/F12/Neurobasal
Main protein source: Albumine
Serum concentration: 0 %
N2 %
Leukaemia Inhibitory Factor
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
See Bredenkamp at al. 2019, doi: 10.1016/j.stemcr.2019.10.009
Transcriptome Characterisation
Differentiation Potency
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
Protocol or reference
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Protocol or reference
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Protocol or reference

Microbiology / Virus Screening

Mycoplasma Negative


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Passage number: >10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)