Establishment of TUSMi008-A, an induced pluripotent stem cell (iPSC) line from a 76-year old Alzheimer's disease (AD) patient with PAXIP1 gene mutation

Summary

A 76-year old Alzheimer's disease (AD) female patient donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wang Y, Zhang J, Hu J, Li G, Lei Y, Zhao J
Journal Stem cell research
Publication Date 2019 Jan 26;36:101391
PubMed 30831521
DOI 10.1016/j.scr.2019.101391

Research Projects

Cell Lines